- Reduced variability during sample preparation
- Radically improved sample yield
- Cryo-EM实验室中工作流的效率提高
体外
Reproducible and Reliable Sample Preparation for single particle analysis
Benefits of the VitroJet:
- Automation for standardizing sample preparation
- Ethane jetting for making vitreous samples with amorphous ice every time
- Blotless technology with consistent and customizable ice thicknesses
- Compatible with multiple grid types
- Pin-printing technology for consistent material deposition onto the grid
- Dew point controlled – no evaporation or condensation after deposition
- 内置血浆清洁剂，以保持网格的一致性
- No dry room required
The VitroJet is a automated solution that aids CryoEM researchers by removing the variability that’s typically associated with sample prep and provides users with a method that is consistent and reproducible. Using pin-printing technology with sub-nanoliter volume sample deposition, and ethane jetting for vitrification, the VitroJet substantially reduces waste and ensures quality samples with customizable ice thickness on every grid.
CERES ICE DEFENSE SYSTEM
减少污染并最大化冷冻的薄片产量
- Reduces amorphous ice contamination
- 在转移期间，在样品上保持长达30分钟的样品状态
- 专用无水样品准备区
- 解锁Fib-Sems上的完整批量研磨功能
在冷冻EM工作流程中，保持样品玻璃化并没有冰污染是一个普遍的挑战。CERES ICE防御系统由三个独特的组件组成，即CERES CERES CLEAN站，Vitrilock和Ice Shield，它们精心设计，以最大程度地减少工作流程中的每一步，分别将冰污染 - 样品制备，传输和双光束铣削。这些强大的工具共同有助于提高高质量薄片的产量，因此用户可以花时间分析数据，而不是对样品的冰层增长进行故障排除。
流星
Instant CLEM with guided milling to maximize ROI’sin final lamella
- Three-fold improvement in sample yield thanks to Z-stacking and deconvolution for targeted z-plane milling
- Upgrade for Aquilos 1 & 2 Microscopes as well as Zeiss, Tescan, and JEOL FIB-SEMs
- In-situ design for simplified correlation of EM and LM micrographs and post-milling sample verification
- Multiple high NA objective (up to 0.9 NA) and sensitive camera (up to 95% QE) options for the widest variety of fluorescence based experiments
Cryo-ET provides users with an excellent opportunity to view proteins and complexes in-situ but identifying these areas within a large volume is very difficult. A reliable method for locating and targeting regions of interest (ROI’s) is necessary to increase throughput and efficiently mill lamellae. Additionally, higher numbers of lamella are often needed to create higher resolution 3D reconstructions. these fluorescence-containing lamellae, a is necessary. The METEOR is an in-situ fluorescence light microscope that can be integrated to a cryo-FIB to identify and verify region of interest during and after milling. The METEOR is an elegant retrofit to microscopes like the Aquilos 1 and 2. It’s also compatible with FIB-SEMs from JEOL, Tescan, and Zeiss.
流星(fluorescent light microscope) image of a 1 um thick lamella of yeast overexpressing eGFP-Edge 1 with the SEM image overlaid.
Image courtesy of A. Bieber, C. Capitanio and O. Schioetz (MPI Bioschemistry).